Seminario impartido por Prof. Rubén Fernández Busnadiego

 Protein aggregation is a hallmark of many neurodegenerative diseases, including Huntington’s, Parkinson’s and amyotrophic lateral sclerosis. However, the mechanisms linking aggregation to neurotoxicity remain poorly understood, partly because only limited information is available on the native structure of protein aggregates inside cells. We address this pressing issue utilizing the latest developments in cryo-electron tomography (cryo-ET). We use cryo-focused ion beam to prepare thin lamellas of vitrified cells containing protein aggregates, and subsequently image them in three dimensions by cryo-ET. This allows us to analyse aggregate structure within pristinely preserved cellular environments and at molecular resolution [1, 2, 3]. Here, I will discuss how our latest results shed new light into the cellular mechanisms of neurodegeneration. References [1] Bäuerlein et al. and Fernández-Busnadiego, Cell (2017) 171 (1), 179-187 (link) [2] Guo et al. and Fernández-Busnadiego, Cell (2018) 172 (4), 696-705 (link) [3] Trinkaus et al. and Fernández-Busnadiego, Nat Comm (2021) 12 (1), 2110 (link)

Fecha del seminario: 18/05/2023 12:00
Lugar del seminario: Instituto de Química Física Blas Cabrera
Ponente del seminario: Dr. Rubén Fernández Busnadiego