News
Vinaora Nivo Slider 3.x

In its 88-year story, the mission of our institute has been to carry out excellence research in fundamental and applied physical chemistry, contributing to the scientific training of several generations of researchers at the highest level. Our vision is to be an international reference in multidisciplinary research focused on the resolution of the present challenges of our society in the fields of health, biotechnology, new materials, and environment.

Intranet

Today

No events

Upcoming events

No events
March 2024
M T W T F S S
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

Since ancestral time mankind has tried to modify plants and animals to obtain an offspring that could adapt to our needs. In this way farmers have selected more efficient hens to produce a larger number of eggs,

more resistant plants, faster and resistant horses or cows that produce a larger amount of milk. Our ability to modify organisms has changed dramatically with the introduction of genome editing. Thanks to the discovery of new genome modifying enzymes we can perform a safer and faster manipulation to edit a genome.

I will address the molecular mechanism that control specificity and cleavage of one of these tools, Cpf1, a single RNA-guided endonuclease of class 2 type V CRISPR-Cas system, which is emerging as a powerful genome editing tool. To provide insight into its DNA targeting mechanism, we have determined the crystal structure of Francisella novicida Cpf1(FnCpf1) in complex with the triple strand R-loop formed after target DNA cleavage. The structure reveals a unique machinery for target DNA unwinding to form a crRNA-DNA hybrid and a displaced DNA strand inside FnCpf1. Our study reveals a singular working model of RNA-guided DNA cleavage by Cpf1, opening up new avenues for engineering this genome modification system. 

SEMINAR’S DATE, TIME AND PLACE: 20 December 2017. 12:00. Assembly Hall

SPEAKER: Guillermo Montoya

ABSTRACT