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Events Calendar

Seminar: Bacterial viruses with a ZTGC genome
Wednesday, 16. June 2021, 16:00
Contact José Miguel Mancheño

Cells have two purine pathways that synthesize adenine and guanine ribonucleotides from phosphoribose via inosylate. A chemical hybrid between adenine and guanine, 2-aminoadenine (Z), replaces adenine in viral DNAs.

Cells have two purine pathways that synthesize adenine and guanine ribonucleotides from phosphoribose via inosylate. A chemical hybrid between adenine and guanine, 2-aminoadenine (Z), replaces adenine in the DNA of the cyanobacterial virus S-2L. We show that S-2L and Vibrio phage PhiVC8 encode a third purine pathway catalyzed by PurZ, a distant paralog of succinoadenylate synthase (PurA), the enzyme condensing aspartate and inosylate in the adenine pathway. PurZ condenses aspartate with deoxyguanylate into dSMP (N6-succino-2- amino-2ʹ-deoxyadenylate), which undergoes defumarylation and phosphorylation to give dZTP (2-amino-2ʹ-deoxyadenosine-5ʹ-triphosphate), a substrate for the phage DNA polymerase. Crystallography and phylogenetics analyses indicate a close relationship between phage PurZ and archaeal PurA enzymes. Our work elucidates the biocatalytic innovation that remodeled a DNA building block beyond canonical molecular biology.

Fecha del seminario: 16/06/2021 16:00

Lugar del seminario: Sesión Zoom IQFR

Ponente del seminario: Pierre Alexandre Kaminski

Abstract

 

Location Sesión Zoom IQFR